Background
High-throughput
Conclusions
The AAV-based system extends the dual-reporter technology to more cell types, allowing for cost-effective and high throughput applications.
Results
We first design and construct the AAV-based reporter system. We then validate the versatility and specificity of this system in monitoring and quantifying two important cell signaling pathways, inflammation (NFκB) and cell growth and differentiation (AP-1), in cultured HEK293 and MCF-7 cells. Our results demonstrate that the AAV reporter system is both specific and versatile, and it can be used in two common experimental protocols including transfection with plasmid DNA and transduction with packaged viruses. Importantly, this system is efficient, with a high signal-to-background noise ratio, and can be easily adapted to monitor other common signaling pathways. Conclusions: The AAV-based system extends the dual-reporter technology to more cell types, allowing for cost-effective and high throughput applications.