Abstract
ZCCHC10 (zinc finger CCHC-type containing 10) has been shown to play a tumor suppressive role in acute myeloid leukemia (AML), but the underlying mechanism is not clear. Many ZCCHC proteins are involved in RNA metabolism, such as synthesis, splicing and stability of RNA. To investigate the role of ZCCHC10 in RNA metabolism, we performed PacBio single-molecule long-read isoform sequencing (Iso-seq) and Illumina short-read RNA sequencing (RNA-seq) on ML-2 cells stably overexpressing ZCCHC10 (designated ML2T) and stably overexpressing an empty vector (designated ML2C). The Iso-seq data consists of full-length transcripts from ML2T and ML2C and can be used by researchers to identify mRNA isoforms and decipher the events of alternative processing, including alternative transcription initiation, alternative splicing, and alternative polyadenylation. The RNA-seq data provides the transcript profiles from ML2T and ML2C and can be used by researchers to study the effects of overexpression of ZCCHC10 on gene expression. Integrated analysis of RNA-seq and Iso-seq datasets can reveal the impact of ZCCHC10 overexpression on alternative processing in AML.