Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques

通过 RNAscope® 原位杂交和免疫组织化学技术检测 SARS-CoV-2

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作者:Mariano Carossino, Hon S Ip, Juergen A Richt, Kendra Shultz, Kimberly Harper, Alan T Loynachan, Fabio Del Piero, Udeni B R Balasuriya

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

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