Protocol for specific induction of apoptosis or necroptosis in established murine tumors

We present a protocol to study cancer cell death in vivo using mouse tumor models by generating cell lines that allow for the induction of "pure" apoptosis or necroptosis via an inducible dimerizer system. We describe steps for lentiviral transduction and flow cytometry-based optimization of the death induction schedule. We then detail optional procedures for "intradermal vaccination" and for tracking antigen-specific CD8+ T cell responses. For complete details on the use and execution of this protocol, please refer to Hänggi et al.1.