Errata: Molecular survey of infectious bronchitis virus on poultry farms in Gifu Prefecture, Japan from 2021 to 2022 by RT-PCR with an enhanced level of detection sensitivity for the S1 gene

Harunobu SAITO, Keisuke NAKAGAWA, Yuko KITAMURA, Keisuke KUWATA, Eiji TANAKA Vol. 84, No. 9 (2022), pp. 1158-1159, the primer sequence should have been as follows: Error: RNAs from the samples collected were extracted by using TRIzol LS reagent (Invitrogen, Carlsbad, CA, USA) and RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany) following the instructions in the manufacturers' product manuals. RT-PCR was performed using the One-Step RT-PCR Kit (TaKaRa Bio Inc., Kusatsu, Japan) and IBV-N gene specific primers (IBV-N forward, 5'-AGCACCCTTAGCAGCAACCC-3'; IBV-N reverse, 5'-ATCTTCAGTTTVGGAGGTAA-3') [25]. Correction: RNAs from the samples collected were extracted by using TRIzol LS reagent (Invitrogen, Carlsbad, CA, USA) and RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany) following the instructions in the manufacturers' product manuals. RT-PCR was performed using the One-Step RT-PCR Kit (TaKaRa Bio Inc., Kusatsu, Japan) and IBV-N gene specific primers (IBV-N forward, 5'-AGCACCCTTAGCAGCAACCC-3'; IBV-N reverse, 5'-CCCGCGTGTACCTCTCTAGT-3'), which were originally designed to locate the region within the N gene with the highest degree of conservation) [25].