Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major)

真鲷(Chrysophrys major)铁调素基因的克隆、鉴定和表达分析

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作者:Song-Lin Chen,Mei-Yu Xu, Xiang-Shan Ji, Guo-Cai Yu, Yang Liu

Abstract

A cDNA encoding hepcidin was isolated from a library of cDNA from spleen of red sea bream (Chrysophrys major) by expressed sequence tag analysis. The expression of the hepcidin mRNA in various tissues was examined. Challenge of red sea bream with Escherichia coli DH5alpha elevated hepcidin mRNA levels in spleen, gill, liver, and intestine.

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