Abstract
Advancing immunosensing technologies hinges on the development of next-generation surface functionalization methods, as the precise anchoring of antibodies on transducer interface is essential for achieving high sensitivity and selectivity. Among the diverse methodologies explored, bioengineered materials have shown significant potential to improve antibody orientation, stability, and functional performance. In this study, we present a chimeric protein created by fusing the adhesive Class I hydrophobin Vmh2 from Pleurotus ostreatus, with the Fc-binding region of protein A from Staphylococcus aureus (SpA). This fusion protein spontaneously adheres on gold nanoparticles (AuNPs) without requiring chemical modification, forming a robust bio-interactive layer for antibody attachment. The platform's adaptability and effectiveness were assessed using an immunoglobulin specific to a fungal laccase to establish the performance of the system, and antibodies against two clinically significant targets- mesothelin, a tumor-associated glycoprotein, and the SARS-CoV-2 spike protein- to showcase the diagnostic potential of the system. A two-step method based on the induced aggregation of the AuNps not bound to the analyte allows underscoring the platform's promise in biosensing applications. Overall, this approach represents a sustainable, versatile, and low-cost route for fabricating biologically active surfaces, with wide-ranging relevance in medical diagnostics, environmental analysis, and biotechnological innovation.
Keywords:
AuNPs aggregation; colorimetric biosensor; functional amyloid proteins; localized surface plasmon resonance; surface functionalization.