Abstract
Here, we present a protocol for studying infections caused by pathogens associated with intrauterine complications-Listeria monocytogenes, Plasmodium falciparum, and Toxoplasma gondii-using an ex vivo placental explant model. We describe procedures for pathogen culture, preparation of placental explants using an air-liquid interface system, and infection assays. We detail procedures for tissue dissociation and CD45+ cell enrichment. This protocol allows short-term placental infection studies. For complete details on the use and execution of this protocol, please refer to Hoo et al.1.