Abstract
Introduction:
Colorectal cancer (CRC) is a molecularly diverse disorder arising from gradual accumulation of genetic and epigenetic changes giving interest in characterization of genetic alterations and microsatellite instability (MSI) for identification of new personalized therapeutic targets.
Objectives:
Evaluating the incidence of somatic mutations in clinically relative signaling pathways involved in CRC tumorigenesis that include mainly Wnt and MAPK pathways together with MSI and gut microbiota.
Methods:
Twenty four CRC patients were enrolled in the study. Cancer hotspot V2 panel was tested using targeted NGS on MiSeqDx device in addition to MSI identification, also gut microbiota was detected using conventional techniques. Patients received FOLFOX regimen in addition to Xeloda especially in early stages.
Results:
Non-synonymous genetic variants were detected inTP53, PIK3CA, KDR, KIT, APC, FGFR3 and MET. Twelve patients (50%) had MSI-LO, 25% had MSI-HI and 20.8% were MSS. Missense mutations in PIK3CA, TP53, and KDR were identified in 2, 3, and 2 patients with MSI-HI status, respectively. In MSI-LO, missense alterations in PIK3CA, TP53, KIT, and KDR were detected in (6, 11, 3, 2 cases, respectively). More than 50% of examined patients revealed mixed GIT flora, 18.8% of patients had E. Coli, 12.5% of patients showed Klebsiella spp. and only 6.3 % of patient revealed Proteus spp. H. pylori antigen was detected in 37.5%of patients and Blastocystis hominis cysts in only 4 patients.
Conclusion:
CRC genetic mutational statuses as well as contributing environmental stress factors such as gut microbiota dysbiosis are prognostically crucial, associated with high risk potential of gene-environment interactions based on machine learning.
Keywords:
Activating mutations; Colorectal cancer; Gut microbiota & protists infections; Microsatellite instability; Next generation sequencing.