Abstract
The complete mitochondrial genome sequence of Bufotes zamdaensis was reported in the present research by shotgun sequencing. The total length of mitogenome is 17,189 bp, and contains 13 protein-coding genes, 22 tRNA genes, two ribosome RNA genes, and one D-loop region. All gene conformations were consistent with the gene arrangement in vertebrates. Most of the genes of B. zamdaensis were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand. Phylogenetic analysis of B. zamdaensis and 12 other closely amphibian species was reconstructed using Maximum-likelihood methods. The sequences of B. zamdaensis were clustered in genus Bufotes. The phylogenetic analyses based on these mitogenomes presented here will be useful to further insights on the evolutionary relationships of Bufonidae.