Abstract
Nuclear Factor Y C (NF-YC) transcription factors (TFs) are central regulators of plant development and stress adaptation. However, there remains a gap in identifying NF-YC gene family members in blueberry (Vaccinium corymbosum), a globally significant fruit crop renowned for its nutritional value and good adaptability. In this study, a total of 31 NF-YC genes (designated VcNF-YC1-31) were identified in the blueberry genome, and their basic physicochemical properties, gene structures, motif patterns, and conserved domains were investigated using bioinformatic methods. The cis-acting elements in the promoters of VcNF-YCs were mainly enriched in phytohormone signaling, metabolism, and stress response. qRT-PCR analysis showed that VcNF-YCs were expressed at higher levels in leaves than in roots and stems. Transcriptional profiling revealed rapid upregulation of 24, 25, and 16 VcNF-YC genes upon ABA, salt, and cold treatments, respectively, indicating stress-specific induction patterns. The results of the yeast transformation assay revealed that VcNF-YC10 and VcNF-YC15 lacked transcription-activating activity. The results of tobacco leaf injection revealed that these two TFs were localized in the nucleus. These findings indicate the potentially important roles in abiotic stress responses of blueberry, offering potential targets for molecular breeding to enhance plant resilience.