Genetic control of seed iron and zinc concentration in Rwandan common bean population revealed by the Genome Wide Association Study (GWAS)

全基因组关联研究(GWAS)揭示了卢旺达普通豆种群种子铁锌浓度的遗传控制机制

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作者:Floride Mukamuhirwa,Kenta Shirasawa,Ken Naito,Edouard Rurangwa,Viateur Ndayizeye,Alphonse Nyombayire,Jean Pierre Muhire,Mahalingam Govindaraj,Norikuni Ohtake,Keiichi Okazaki,Moeko Okada,Eigo Fukai

Abstract

Common bean (Phaseolus vulgaris L.) is one of the most abundantly consumed legume crops as foods worldwide. In many African countries, this crop is an important staple food because of its rich nutrients. The Great Lakes region of Central Africa, which includes Rwanda, the nation with the highest per capita consumption of common beans worldwide, is known to be a center of common bean diversity in Africa. Increasing the amount of iron and zinc in common bean for biofortification has been a key breeding goal in Rwanda and other countries. In this study, using 192 accessions, including local landraces from Rwanda, breeding materials, released varieties, and others, we performed genome wide association studies (GWAS) to determine the loci governing those traits in addition to other agronomic traits. We identified a locus that was strongly associated with seed zinc concentration and candidate genes. The information might be a great help for marker-assisted breeding of this trait in common bean.

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