Abstract
Background: Glioblastoma multiforme (GBM), the most prevalent and challenging-to-diagnose brain tumor, is a highly lethal and largely untreatable form of brain cancer. Circular RNAs (CircRNAs) and microRNAs (miRNAs) may serve as biomarkers for GBM diagnosis and treatment. Circ_100290 and miR-205-5p play a key role in gene regulation, tumor progression, and cellular communication, making them valuable biomarkers for GBM, but their precise role in GBM remains unclear and requires further investigation. Methods: Bioinformatics analyses were conducted to explore interactions between miR-205-5p and circ_100290, their functional roles, and associated pathways. Additionally, we assessed the differences of miR-205-5p and circ_100290 between tumor and normal samples by analyzing the quantitative real-time PCR. Results: Bioinformatics analysis revealed a network association of miR-205-5p and circ_100290 to key proteins (PTEN, SMAD4, CDK19, ELAVL1, HNRNPK, DDX5, AGO2, and EIF4A3) and pathways, including Wnt signaling, highlighting their roles in splicing and gene expression regulation and the regulation of tumor development and metastasis. QRT-PCR analysis revealed a significant downregulation of miR-205-5p and an upregulation of circ_100290 compared to normal tissues. Notably, the downregulation of miR-205-5p was associated with increased tumor volume and lobar tumor localization, whereas the upregulation ofcirc_100290 correlated with IDH1 gene mutations in GBM patients. Conclusions: Our study highlights miR-205-5p downregulation and circ_100290 upregulation in GBM patients which underscore their diagnostic potential and cooperative role in GBM progression, offering novel insights for therapeutic targeting.