New microsatellite markers for Tricyrtis macrantha (Convallariaceae) and cross-amplification in closely related species

铃兰科植物大花油点草(Tricyrtis macrantha)的新微卫星标记及其在近缘物种中的交叉扩增

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作者:Noriko Ohki,Hiroaki Setoguchi

Abstract

Premise of the study: Microsatellite markers were characterized in Tricyrtis macrantha (sect. Brachycyrtis, Convallariaceae), a vulnerable species endemic to Japan, to investigate its genetic diversity and population structure. • Methods and results: Eleven microsatellite markers were developed. The number of alleles per locus ranged from one to seven, and genetic diversity per locus ranged from 0.142 to 0.812. Four markers were successfully amplified in other species of sect. Brachycyrtis (T. ishiiana, T. ishiiana var. surugensis, and T. macranthopsis). • Conclusions: The microsatellite markers can be used to investigate the genetic diversity and population structure of the vulnerable and endangered species of Tricyrtis sect. Brachycyrtis, to aid in the development of conservation strategies for each species.

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