Significance
This manuscript focused on the in situ dual-crosslinked nanoparticles for tumor targeting pDNA delivery. The novel system is prepared by in situ shielding HA-CHO on PEI/DNA complexes. The electrostatic crosslink formed between carboxyl groups on HA-CHO and amine groups on PEI as well as the reaction between aldehyde groups on HA-CHO and amine groups on PEI contributes to the chemical crosslink. By introduction of HA-CHO on PEI/DNA complexes, they show promoting colloidal stability, enhanced cellular uptake and tumor targeting ability. The in vivo experiments further confirm the excellent ability of long circulation and tumor accumulation. Accordingly, HA-CHO2/PEI/DNA has great potential for tumor targeting antitumor therapy.
Statement of significance
This manuscript focused on the in situ dual-crosslinked nanoparticles for tumor targeting pDNA delivery. The novel system is prepared by in situ shielding HA-CHO on PEI/DNA complexes. The electrostatic crosslink formed between carboxyl groups on HA-CHO and amine groups on PEI as well as the reaction between aldehyde groups on HA-CHO and amine groups on PEI contributes to the chemical crosslink. By introduction of HA-CHO on PEI/DNA complexes, they show promoting colloidal stability, enhanced cellular uptake and tumor targeting ability. The in vivo experiments further confirm the excellent ability of long circulation and tumor accumulation. Accordingly, HA-CHO2/PEI/DNA has great potential for tumor targeting antitumor therapy.