Recent Advances in Nanodelivery Systems Based on Extracellular and Intracellular Reprogramming Strategies for Enhanced Therapy of Atherosclerosis

基于细胞外和细胞内重编程策略的纳米递送系统在增强动脉粥样硬化治疗中的最新进展

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Abstract

Atherosclerosis (AS) represents a chronic immunoinflammatory disorder characterized by lipid metabolism dysregulation, predominantly affecting medium and large arteries. During the initial phase of the disease, endothelial cells (ECs) undergo mesenchymal transformation, subsequently releasing various adhesion molecules and chemokines that facilitate the recruitment of monocytes and neutrophils (NEs) to developing plaque sites. Under sustained inflammatory stimulation, NEs transform into neutrophil extracellular traps (NETs), monocytes differentiate into pro-inflammatory macrophages, and vascular smooth muscle cells (VSMCs) exhibit phenotypic switching, and adopt macrophage-like characteristics. These inflammatory cells release excessive inflammatory factors and reactive oxygen species (ROS), thereby amplifying the inflammatory cascade. Concurrently, these activated macrophages and VSMCs internalize oxidized low-density lipoprotein (ox-LDL) particles, promoting foam cell's generation and plaque's formation. Given the critical role of the activation of various inflammatory cells and the accumulation of inflammatory mediators in progression of the disease, nanodelivery systems (NDSs) have shown remarkable promise in treating AS, attributed to their excellent cell-specific targeting capabilities and regulation capabilities for inflammatory mediator. This paper comprehensively reviews the progress of NDSs based on intracellular and extracellular reprogramming strategies for the therapy of AS, emphasizing their role in reversing EC's phenotypes, monocytes' reprogramming, macrophages' repolarization, inhibition of Nes' recruitment, suppression of VSMCs' proliferation, modulation of inflammatory mediators, reprogramming of lipid's metabolism, and scavenging of ROS to enhance therapeutic efficacy. We further elaborate on the advantages, challenges, and opportunities of NDSs in enhancing intracellular and extracellular reprogramming strategies, aiming to deepen these strategies and their potential applications in the therapy of AS.

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