Conditioned media from adipose-derived mesenchymal stem cells improves movento induced lung damage in male rats

脂肪间充质干细胞条件培养基可改善雄性大鼠Movento诱导的肺损伤

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Abstract

Insecticides are chemicals agents used to kill insects to increase crop growth. But they can be toxic to humans and wildlife. Movento is an insecticide which is highly toxic to humans and animals by oral, dermal, and inhalation routes. This research is to assess the pulmonary impacts of this insecticide, which have not been explored in previous research. Wistar rats were adopted to four groups. 1- Control (CTL), 2- Movento (M), 3- M in conjunction with Basal medium (BM), 4- M in conjunction with Conditioned medium (CM). For evaluation of the degree of injury and fibrosis, the Hematoxylin and Eosin (H&E) and Masson's trichrome stainings were done respectively. Oxidative stress markers and inflammatory mediators were analyzed utilizing appropriate assays. In rats administered Movento's insecticide, antioxidant markers diminished in comparison to the control group; however, curation with CM markedly elevated them relative to the M and M + BM groups. Movento elevated histological score, fibrosis, MDA, TNF-α, and IL-6 compared to the control group, whereas CM remarkably diminished these parameters in relation to the M and M + BM groups. Conditioned media derived from mesenchymal stem cells (CM-MSC) may function as a therapeutic intervention for lung illnesses induced by movento in rat lungs. It may also serve as a potential treatment of alleviating lung injuries in farmers who are exposed to agricultural pesticides.

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