Abstract
BACKGROUND: Liver X receptors (LXR) play an essential role in the regulation of cholesterol metabolism, and their activation increases ABCG5 and ABCG8 gene expression for the improvement of cholesterol excretion from the body during reverse cholesterol transport (RCT). The aim of this study was to investigate the effects of high-intensity interval (HIT) and low-intensity continuous (LIT) trainings on gene expression of these substances after a high-fat diet in Wistar rats. METHODS: Fifteen male Wistar rats were divided into 3 groups: control group (n = 5), HIT exercise group (n = 5), and LIT exercise group (n = 5). All groups were fed a high-fat diet for 13 weeks, and the HIT and LIT groups performed the specific training program. The expression of LXRβ, ABCG5, and ABCG8 genes was measured after the training period. RESULTS: Data analysis showed significantly higher levels of LXRβ, ABCG5, and ABCG8 gene expression in the HIT and LIT groups compared to the control group (P ≤ 0.05). CONCLUSION: HIT and LIT trainings after a high-fat diet have beneficial effects on RCT, preventing heart attacks. Additionally, HIT training may have a greater effect on cholesterol excretion during the reverse cholesterol transport mechanism than LIT.