CRISPR-based m(6)A modification and its potential applications in telomerase regulation

基于CRISPR的m(6)A修饰及其在端粒酶调控中的潜在应用

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Abstract

Telomerase determines cell lifespan by controlling chromosome stability and cell viability, m(6)A epigenetic modification plays an important role in the regulation of telomerase activity. Using CRISPR epigenome editing to analyze specific m(6)A modification sites in telomerase will provide an important tool for analyzing the molecular mechanism of m(6)A modification regulating telomerase activity. In this review, we clarified the relevant applications of CRISPR system, paid special attention to the regulation of m(6)A modification in stem cells and cancer cells based on CRISPR system, emphasized the regulation of m(6)A modification on telomerase activity, pointed out that m(6)A modification sites regulate telomerase activity, and discussed strategies based on telomerase activity and disease treatment, which are helpful to promote the research of anti-aging and tumor related diseases.

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