Splice choices at the threshold of activation: Alternative splicing fine-tunes Notch signaling in muscle stem cells

Alternative splicing events have emerged as a rapid regulatory layer in gene expression. Lin et al. demonstrate that alternative splicing is widespread during muscle stem cell activation. Its functional importance is illustrated through an RNA-binding fox 1-homolog 2 (RBFOX2)-dependent splice choice in the Notch regulator Numb, showing how inclusion of a single exon can tune Notch signaling to regulate the transition from quiescence to activation.