Abstract
Genome annotation, alignment and phylogenetics are at the centre of most studies in evolutionary genomics. These techniques function best when rooted in prior work. Genes are mined from new genomes using evidence from old gene models. These genomes are aligned to well-worn references to create matrices for tree reconstruction. Trees are often populated with well-characterised genomes to add context to the newly sequenced. Genome inference traces a line back to model organisms, yoking the analysis of new genomes to layers of previous knowledge. Here, we present an alternative approach that uses unannotated and unaligned sequence to understand the information diversity of sequence ensembles. Any set of genomes can comprise our sequence ensemble. In a pandemic context, a sequence ensemble might be clinically isolated strains from one day. In a systematic context, a sequence ensemble could be the pangenome available for a clade. The normal bioinformatics playbook would have us align. But we instead compress. A sequence ensemble that compresses easily contains lower information diversity. For pandemics, we can use curves of information diversity to trace genomic novelty and monitor selective sweeps in new strains. For systematics, we can calculate compressibility quickly across all known bacterial taxa, levelling the criteria for species across clades. If we tolerate data loss, we can go one step further and capture structural evolution as we compress. Our approach sacrifices a lot. We skip many of the products of modern bioinformatics like variation anchored to known genes or genome alignment to prescribed references or pangenome graphs. But we gain speed, breadth and the ability to rapidly respond to novelty.