Abstract
OBJECTIVE: Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV(-pos) DLBCL) is a recently identified entity. Data regarding outcome to frontline immuno-chemotherapy are conflicting. Although the prognostic impact of the tumour microenvironment (TME) in EBV(-neg) DLBCL is well-established, it remains untested whether the TME influences survival in EBV(-pos) DLBCL. There are no data with new digital gene expression technologies that simultaneously interrogate the virus, B cells and the tumour microenvironment (TME). METHODS: We used the NanoString™ platform in a population-based cohort of 433 patients to establish if the technology could detect EBV in the tumour biopsies and to investigate the influence that EBV has on the complex tumour microenvironment of DLBCL. RESULTS: Incidence of EBV(-pos) DLBCL was 6.9% with 5-year survival of 65% vs 82% in EBV(-neg) DLBCL (P = 0.018). EBV(-pos) tissues had similar expression of T-cell genes compared to EBV(-neg) DLBCL but higher levels of the antigen-presenting molecule B2M. This was countered by elevated PD-L1, PD-L2, LAG3 and TIM3 immune checkpoints and a higher CD163/CD68 "M2" macrophage score. CONCLUSION: In EBV(-pos) DLBCL, the TME is immuno-tolerogenic and may explain the poor outcomes seen in this subtype of DLBCL.