Abstract
BACKGROUND: Air pollution is a significant public health concern, increasingly recognized for its association with adverse health outcomes including neurodegenerative and neuroinflammatory conditions. The present study aimed to characterize plasma levels of key biomarkers related to neurodegeneration and neuroinflammation among middle-aged to elderly adults living in areas designated as critically polluted. METHODS: A total of 202 adults, aged 41 to 60 years, residing in CPA (CEPI > 70) for over ten years were recruited in the study. The exposures of air pollutant were measured as per the established protocols by CPCB. The plasma levels of neurodegenerative markers (Aβ((1-42)), Total τ, α-Synuclein, BDNF and GFAP) were estimated using commercially available ultra-sensitive ELISA kits. The data analysis was performed through mean and standard deviation, percentile distribution and multivariate logistic regression using SPSS 26.0. RESULTS: This study confirmed the elevated PM(2.5) levels at the study location exceeding the regulatory limits. Women exhibited relatively higher Amyloid Aβ(1-42), α-Synuclein and GFAP levels, while men exhibited relatively higher Total τ, & BDNF levels. Further, older participants (aged 50 - 60 years) exhibited higher levels of all markers but α-Synuclein, as compared to the younger peers (aged 40 - 50 years). A weak positive trend (p = 0.08) was observed for α-Synuclein with prolonged exposure. CONCLUSION: This study is among the first community-based investigations in India to assess plasma levels of neurodegenerative and neuroinflammatory biomarkers in apparently healthy adults chronically exposed to high ambient air pollution. By integrating chronic exposure data from a Critically Polluted Area (CEPI > 70) with biomarker profiling, the study offers early insights into potential neurobiological alterations associated with environmental pollutants, highlighting sex- and age-specific vulnerabilities. These findings emphasize the importance of considering environmental influences in neurodegenerative disease research and the potential need for tailored health interventions.