Abstract
Prussian Blue Nanoparticles (PBNPs)-based dual-mode immunochromatographic assays (ICAs) were developed for the detection of norfloxacin (NOR) in animal-derived foods. The assays used the inherent bright blue color and peroxidase-like catalytic activity of PBNPs. The visual limit of detection (vLOD) of the developed ICAs were both determined to be 1 ng mL(-1). The calculated limit of detection (cLOD) of the ICA based on the inherent bright blue color of PBNPs was 0.5 ng mL(-1), while that of the ICA using the peroxidase-like catalytic activity of PBNPs was 0.34 ng mL(-1). Analysis of spiked samples demonstrated that NOR could be effectively detected in pork, chicken, beef, sea bass and shrimp, with recoveries ranging from 80.43 % to 106.33 %. The results showed strong correlation with those obtained by HPLC, with R(2) values of 0.9653 and 0.9715, respectively. Therefore, this study established a sensitive, accurate, rapid and cost-effective method for detecting NOR in animal-derived foods.