Abstract
Nuclear staining using fluorescent dyes is crucial for cytological studies in plants. However, few fluorescent dyes are suitable for live-cell imaging of the nucleus. Here, we demonstrate that dimidium bromide (DimBr), a commercially available fluorescent dye, can be used to stain the nucleus (nucleolus and nucleoplasm) in living plant cells. DimBr emits peak fluorescence at 600 nm at an excitation wavelength of 525 nm, making it well suited for use with green fluorescent protein. DimBr staining can be used in various plant species and allows time-lapse observation of the nucleus. Therefore, DimBr can be used to visualize the nucleus in living plant cells, making it a valuable tool for plant cell biology.