Abstract
RNA-sequencing provides rich transcriptomic data about cell types, and new methods allow characterization at the single-cell level. For neurons, single-nucleus RNA sequencing (snSeq) is the gold standard, as this technique retains neuron identity. We carried out snSeq on sorted nuclei from C. elegans neurons of Day 1 adult wild-type ( N2 ) and daf-2 mutants, and males and genotypically-matched hermaphrodites. To provide the field with an easily accessible atlas of this information, we created C. elegans Single-nucleus Transcriptomic Atlas of Adult Neurons (CeSTAAN) (https://cestaan.princeton.edu/), described here with examples. CeSTAAN will allow the C. elegans field to access this adult neuron transcriptional information.