Improved and expanded Q-system reagents for genetic manipulations
改进和扩展的 Q 系统试剂用于基因操作
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作者:Olena Riabinina, David Luginbuhl, Elizabeth Marr, Sha Liu, Mark N Wu, Liqun Luo, Christopher J Potter
| 期刊: | Nature Methods | 影响因子: | 36.100 |
| 时间: | 2015 | 起止号: | 2015 Mar;12(3):219-22, 5 p following 222. |
| doi: | 10.1038/nmeth.3250 | 种属: | Goat |
| 靶点: | IgG Fc | 研究方向: | 信号转导 |
Abstract
The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.
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