Dynamics of the transcriptional landscape during human fetal testis and ovary development

人类胎儿睾丸和卵巢发育过程中转录景观的动态

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作者:Estelle Lecluze, Antoine D Rolland, Panagiotis Filis, Bertrand Evrard, Sabrina Leverrier-Penna, Millissia Ben Maamar, Isabelle Coiffec, Vincent Lavoué, Paul A Fowler, Séverine Mazaud-Guittot, Bernard Jégou, Frédéric Chalmel

Methods

First trimester fetuses (6-12 PCW) and second trimester fetuses (13-14 and 17 PCW) were obtained from legally induced normally progressing terminations of pregnancy. Total RNA was extracted from whole human fetal gonads and sequenced as paired-end 2 × 50 base reads. Resulting sequences were mapped to the human genome, allowing for the assembly and quantification of corresponding transcripts. Main

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