Elective single embryo transfer in in vitro fertilization cycles with or without preimplantation genetic testing using next-generation sequencing: A randomized clinical trial

在体外受精周期中进行选择性单胚胎移植,无论是否进行植入前遗传学检测(采用二代测序):一项随机临床试验

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Abstract

INTRODUCTION: Preimplantation Genetic Testing (PGT-A) has been increasingly used in IVF to improve embryo selection. However, its clinical utility in patients with a good prognosis remains debated. There is a lack of robust scientific evidence about the benefits of preimplantation genetic testing Next-Generation Sequencing (NGS) on the clinical pregnancy and live birth rates in good-prognosis IVF patients undergoing elective Single Embryo Transfer (eSET) testing. OBJECTIVE: To compare the clinical pregnancy rate after IVF treatment in patients with a good prognosis between groups that receive the transfer of a single embryo with or without pre-implantation genetic embryo analysis. METHODS: This work was a prospective randomized clinical study that evaluated 206 patients to compare the clinical pregnancy rate after the first elective transfer of an embryo, associated or not with pre-implantation genetic embryo analysis using Next-Generation Sequencing (NGS). Patients were randomized into groups: SET group ‒ patients undergoing the transfer of a single embryo (eSET, elective Single Embryo Transfer) without embryo genetic analysis (SET group, n = 103); and NGS + SET group ‒ patients undergoing eSET with embryo genetic analysis (NGS + SET group, n = 103). RESULTS: The clinical pregnancy rate after the first transfer was similar between the two groups (SET: 76.7 % and NGS + SET: 79.6 %; p = 0.613). There was also no statistical difference in live birth rates or abortion rates between the groups analyzed. CONCLUSION: This clinical trial suggests no significant improvement in the embryo genetic analysis in IVF treatments for infertile couples with a good prognosis.

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