Whole-genome sequencing and integrative genomic data-driven profiling of Carbapenems-resistant Klebsiella pneumoniae isolated from human urine samples in the Iraq

对从伊拉克人类尿液样本中分离出的耐碳青霉烯类肺炎克雷伯菌进行全基因组测序和整合基因组数据驱动的分析

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Abstract

Here, we present whole genome sequencing (WGS) data of clinical Klebsiella pneumoniae and K. quasipneumoniae isolates obtained from urine samples collected between October 2024 and January 2025 from patients in hospitals in Baghdad, Iraq. A total of 30 K. pneumoniae isolates were initially recovered and screened using antimicrobial susceptibility testing, from which one carbapenem-resistance isolate (isolate 5) and one carbapenem- susceptible isolate (isolate 6) were selected for genome sequencing. Genomic DNA was extracted, sequenced using the illumine MiSeq platform, and assembled de novo. The resulting genome assembled were annotated using NCBI prokaryotic genome annotation pipeline and further analysed using the PATRIC platform. The dataset includes annotated genome assemblies with identified carbapenemase genes (including blaNDM-5, blaOXA-48, and blaOXA-181), additional antimicrobial resistance determinants, predicted virulence-associated genes, mobile genetic elements, and multilocus sequence typing results. Isolate 5 was assigned to sequence type ST14 (K. pneumoniae), while isolate 6 was assigned to ST7604 (K. quasipneumoniae). These genome data provide a curated genomic resource that can be reused for comparative genomics, antimicrobial resistance surveillance, and phylogenomic analysis of Klebsiella species from clinical setting in Iraq.

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