Abstract
16S rRNA amplicon sequencing of mammary and gut samples (N = 32) from healthy (n = 3) and experimentally induced mastitis mice (n = 13) revealed Lactobacillus-dominated bacteriomes, low archaeal diversity, and significant mastitis-associated shifts. These results provide a foundational data set for understanding mastitis-causing major pathogen-driven dysbiosis, host-microbiome interactions, and disease-specific microbial community dynamics.