Abstract
Accurate molecular tools are essential for estimating zoonotic malaria transmission in Southeast Asia. This study applied ultrasensitive reverse-transcriptase real-time polymerase chain reaction (PCR) to detect zoonotic malaria in febrile patients from health facilities across three mainland districts (Batubara, Tanjung Balai, and Central Tapanuli) and separately on Mursala Island, North Sumatra, Indonesia. Among 64 participants on Mursala, 7 (10.9%) adults had Plasmodium knowlesi infections (including 5 agricultural workers and 2 adults with severe WHO anemia), and 2 (3.1%) adults had Plasmodium vivax infections. All were negative by microscopy and panparasite lactate dehydrogenase rapid diagnostic tests. No P. knowlesi infections were identified among 947 participants from mainland sites; PCR detected confirmed Plasmodium species in 26%, including P. vivax (17.5%) and Plasmodium falciparum (7.5%), with 30% of cases being submicroscopic. No Plasmodium cynomolgi infections were identified. Plasmodium knowlesi transmission is low in North Sumatra; however, it may cause WHO-defined severe malaria. Molecular diagnostics remain crucial for identifying zoonotic malaria and should be integrated into surveillance systems to inform public health control measures.