Abstract
In August 2025, West Nile virus (WNV) was detected for the first time in Belgium through a monitoring programme in wild birds, with three corvids testing positive by RT-qPCR. In September and October, four additional infected birds were identified. Whole genome sequencing classified the strain as WNV lineage 2, consistent with strains circulating elsewhere in Europe. These detections provide evidence of local WNV circulation with important implications for animal and public health preparedness and surveillance during the 2026 mosquito season.