Abstract
Whole-genome sequencing and antimicrobial susceptibility testing were performed on 29 Campylobacter isolates from retail meat in Puerto Rico. Resistance to tetracycline, ciprofloxacin, clindamycin, and florfenicol was observed. Multidrug resistance was linked to co-occurrence of tet(O), gyrA mutations, and cmeABC, highlighting the public health risk of antimicrobial-resistant Campylobacter in retail meat.