A case report of rapid diagnosis of Sporothrix globosa infection using MetaCAP

利用 MetaCAP 快速诊断球形孢子丝菌感染的病例报告

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Abstract

BACKGROUND: Sporothrix globosa (S. globosa) is a significant pathogenic fungus responsible for, causing sporotrichosis. Metagenomics capture (MetaCAP), a high-throughput sequencing technology for pathogen nucleic acid detection based on probe capture, facilitates early diagnosis of S. globosa infections. Herein, we present a case of S. globosa infection diagnosed using MetaCAP. CASE SUMMARY: A 47 year-old female, initially diagnosed with reactive perforating collagenosis, developed epidermal erosion at the affected site after self-applying a poorly air-permeable topical patch purchased online. She subsequently exhibited progressive redness, edema, severe pruritus, and an increase in papules that coalesced into plaques following exposure to decaying wood. She revisited our hospital for further consultation. The diagnosis of sporotrichosis caused by S. globosa was confirmed through a pathological examination of the affected skin tissue and MetaCAP testing. Then, she was treated with itraconazole and naftifine hydrochloride and ketoconazole cream. After a three-month follow-up, the patient's skin rash showed significant improvement. CONCLUSION: A case of S. globosa infection was promptly diagnosed through MetaCAP and effectively treated with itraconazole.

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