Abstract
Biliary tract cancer (BTC) is potentially influenced by metabolic dysregulation, yet previous metabolomic evaluations are limited. To address this gap, we prospectively investigated associations of blood metabolites and BTC risk in the UK Biobank cohort study. We used Cox proportional hazards models to estimate hazard ratios (HRs) and 95% CIs between 249 plasma metabolites per standard deviation (SD) and BTC risk in 232 781 participants. We implemented exploratory factor analyses and evaluated associations between factors and BTC risk. Associations with a P value <.001 were considered statistically significant after multiple comparison adjustments. In a median follow-up of 11.8 years, we identified 268 first primary incident BTC cases. Of 49 biomarkers significantly associated with BTC risk, 12% were fatty acids, and 49%, 31%, and 8% were cholesterol, triglyceride, and phospholipid to total lipids ratios, respectively. Multiple cholesterol ratios were inversely associated with BTC (HR = 0.74; 95% confidence interval (CI), 0.65-0.84; P < 6.0 × 10-6). Conversely, a triglyceride to total lipids ratio was positively associated with BTC (HR = 1.40; 95% CI, 1.22-1.61; P = 2.5 × 10-6). Congruently, a factor high in cholesterol measures and low in triglyceride measures was inversely associated with BTC. Multiple metabolite biomarkers were associated with BTC risk, suggesting metabolism has a substantial role in BTC etiology.