Abstract
Western blotting is a widely used technique for the detection of specific proteins and is a fundamental tool in biomedical research and education. However, conventional chemiluminescent detection systems are expensive and often inaccessible in educational or resource-limited laboratory environments. In this study, we evaluated a low-cost smartphone-based chemiluminescence detection method and demonstrated that it enables reliable qualitative detection of protein expression, highlighting its potential for both educational and basic research applications.