Abstract
RATIONALE: TW-SLIM ion mobility separations have demonstrated exceptional resolution by leveraging long paths with minimal loss. All previously reported experiments have used electrode surfaces which are mirrored to generate symmetrically opposing electric fields for ion confinement. However, work with other planar ion optics indicates this may be unnecessary. This study explores conditions under which separations may be obtained using a SLIM with asymmetric electric fields. METHODS: The asymmetric field configuration was defined by applying a uniform DC potential to all electrodes of the top PCB of a standard TW-SLIM board pair, with no electrode placement modifications. This configuration was simulated in SIMION to assess transmission through the SLIM. A benchtop TW-SLIM instrument outfitted with a Faraday plate detector was modified likewise, so the top PCB had a uniform DC potential applied to all electrodes, while the bottom board was operated normally. RESULTS: Simulations show full ion transmission for four different m/z ion populations over a range of DC biases applied to the "pusher" board. Likewise, the modified benchtop instrument is capable of transmitting, separating, and cycling ions with minimal losses. The effect of pusher strength on separation quality is explored, and comparisons between the standard and modified SLIM are made with respect to resolving the +2 and +3 charge states of neurotensin ions. CONCLUSIONS: A functional IMS instrument using asymmetric confining fields demonstrates additional field modifications may be a means to achieve additional functionality with limited interruption of the analysis. A TW-SLIM PCB specifically designed as a pusher board would benefit from minimized manufacturing cost, simplifying assembly, reducing drive electronics, and improved field consistency.