Combined Genome and Transcriptome Analyses of the Ciliate Schmidingerella arcuata (Spirotrichea) Reveal Patterns of DNA Elimination, Scrambling, and Inversion

纤毛虫 Schmidingerella arcuata(螺旋藻)的基因组和转录组联合分析揭示了 DNA 消除、扰乱和倒位的模式

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作者:Susan A Smith, Xyrus X Maurer-Alcalá, Ying Yan, Laura A Katz, Luciana F Santoferrara, George B McManus

Abstract

Schmidingerella arcuata is an ecologically important tintinnid ciliate that has long served as a model species in plankton trophic ecology. We present a partial micronuclear genome and macronuclear transcriptome resource for S. arcuata, acquired using single-cell techniques, and we report on pilot analyses including functional annotation and genome architecture. Our analysis shows major fragmentation, elimination, and scrambling in the micronuclear genome of S. arcuata. This work introduces a new nonmodel genome resource for the study of ciliate ecology and genomic biology and provides a detailed functional counterpart to ecological research on S. arcuata.

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