Xtricorder: a likelihood-enhanced self-rotation function and application to a machine learning-enhanced Matthews prediction of asymmetric unit copy number

Xtricorder:一种似然增强的自旋转函数及其在机器学习增强的马修斯非对称单元拷贝数预测中的应用

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Abstract

Analysis of crystallographic diffraction data after collection and integration but before phasing gives the crystallographer a `first-look' assessment of data quality and flags potential challenges in subsequent structure determination. We here report the development of Xtricorder, a `first-look' application specifically targeted at likelihood-based phasing. Xtricorder incorporates the full array of analyses previously available in the Phaser codebase, with some enhancements and updates, in a more streamlined and accessible implementation. In addition, Xtricorder offers a likelihood-enhanced self-rotation function. A novel graphical representation of the self-rotation function, the `composite-section diagram', presents the results for user inspection and has the added advantage that, in an adapted form, it is appropriate for training a convolutional neural network to enhance the standard Matthews analysis and double the accuracy of asymmetric unit copy-number prediction. We investigate the usefulness of the likelihood-enhanced self-rotation function in `first-look' analyses, exploring the circumstances under which the self-rotation function results are useful, and discuss the application to AI-generated structure prediction.

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