Abstract
(1) Background: Chitons (Mollusca, Polyplacophora) are relatively primitive species in Mollusca that allow the study of biomineralization. Although mitochondrial genomes have been isolated from Polyplacophora, there is no genomic information at the chromosomal level; (2) Methods: Here we report a chromosome-level genome assembly for Acanthochiton rubrolineatus using PacBio (Pacific Biosciences, United States) reads and high-throughput chromosome conformation capture (Hi-C) data; (3) Results: The assembly spans 1.08 Gb with a contig N50 of 3.63 Mb and 99.97% of the genome assigned to eight chromosomes. Among the 32,291 predicted genes, 76.32% had functional predictions. The divergence time of Brachiopoda and Mollusca was ~550.8 Mya (million years ago), and that of A. rubrolineatus and other mollusks was ~548.5 Mya; (4) Conclusions: This study not only offers high-quality reference sequences for the Acanthochiton rubrolineatus genome, but also establishes groundwork for investigating the mechanisms of Polyplacophora biomineralization and its evolutionary history. This research will aid in uncovering the genetic foundations of molluscan adaptations across diverse environments.