Abstract
Laser speckle contrast imaging (LSCI) is a non-invasive, powerful, and cost-effective imaging technique that has seen widespread adoption across various medical fields, particularly for blood flow imaging. While LSCI provides physicians with valuable insights into changes or occlusions in blood flow, the technique is susceptible to various factors and parameters that can impact measurement sensitivity and signal-to-noise ratio (SNR). These include the scattering of light, which can affect the quality and reliability of the LSCI data. The polarization of light holds significant promise to enhance the performance of LSCI. In this study, we employed polarization manipulation of light to investigate its impact on the performance of LSCI for measuring flow. Focusing on the application of LSCI in microcirculation within capillaries, we examined the effect of polarization control on the technique's flow measurement capabilities using a custom-designed phantom system. This phantom consisted of three tubes with inner diameters of 1.1 mm, 1.6 mm, and 2.8 mm, embedded in a polydimethylsiloxane (PDMS) matrix with optical properties similar to biological tissue. By manipulating the polarization of both the incident and reflected light, alternating between parallel and perpendicular states, we compared the performance of our LSCI system in detecting flow for different tube diameters and depths within the phantom. Our study revealed that while depth is a critical parameter influencing flow detection using LSCI, employing perpendicular polarization (between incident and reflected light) resulted in the lowest measurement error and highest SNR compared to parallel polarization and the absence of polarization control.