Analysis of Differentially Expressed Proteins Involved in Shrimp and Crab Allergies

虾蟹过敏相关差异表达蛋白的分析

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Abstract

BACKGROUND: Shrimp and crab allergies have garnered increasing attention in recent years. Unlike many other food allergies, they are less likely to be outgrown by children and tend to trigger more severe allergic symptoms. The underlying mechanisms that lead to these phenomena have not yet been fully elucidated. METHODS: We used proteomics iTRAQ technology to identify differentially expressed proteins in shrimp and crab allergic patients and normal controls. RESULTS: Ninety differentially expressed proteins, including 82 upregulated proteins and 8 downregulated proteins, were identified. Furthermore, MRC2 was validated to be upregulated in shrimp and crab allergic patients by ELISA. CONCLUSION: These findings have established a comprehensive proteomics map of shrimp and crab allergies, laying the foundation for further analysis of the pathogenesis and regulatory network of shrimp and crab allergies.

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