Abstract
BACKGROUND: The host microbiome is increasingly recognized as a key modulator of brain function and disease progression, yet the role of the oral microbiome in patients with prolonged disorders of consciousness remains underexplored. METHODS: This study characterized oral microbiota differences among pDoC patients (n = 89) in the vegetative state (VS), the minimally conscious state (MCS), and emerging from the MCS (EMCS), with a particular focus on the impact of antibiotic use. We used 16S ribosomal RNA sequencing to profile oral microbiota in patients with different levels of consciousness. RESULTS: β-diversity was significantly reduced in the VS group compared to the EMCS group. Differential abundance analysis identified five taxa (i.e., species Streptococcus danieliae, species Corynebacterium durum, family Lachnospiraceae, species Phocaeicola abscessus, and species Campylobacter showae) that exhibited significant differences between VS and EMCS, suggesting they were potentially involved in regulating oral microbial dysbiosis and brain-microbiome interactions. Antibiotic treatment induced pronounced microbial shifts in the VS group, while no such effect was observed in the MCS or EMCS groups. Prognostic models built using differential and dominant microbiota panels demonstrated strong predictive performance, achieving areas under the curve of 0.820 and 0.920, respectively. CONCLUSIONS: These findings highlight oral microbiome alterations in pDoC and their potential relevance to disease progression, emphasizing the importance of microbiome-informed clinical strategies.