Abstract
Epstein-Barr virus (EBV) has been extensively studied for its associations with autoimmune disorders, various cancers, and neurological diseases. Emerging evidence also links EBV to behavioral and neurophysiological disruptions, potentially mediated through interactions with host's immune and circadian systems. In this study, we investigated the effects of EBV and its DNA on the behavior of Drosophila melanogaster by examining its lifespan, activity, sleep, and circadian rhythms. Both EBV viral particles and EBV DNA showed distinct effects in terms of behavior and survival. Circadian function analysis showed disruptions in several circadian parameters in EBV-injected flies, whereas EBV DNA-injected flies displayed defects in sleep behavior. Our findings suggest that EBV may impact circadian mechanisms, thereby enhancing our understanding of the effects of viral infections on circadian and behavioral systems and establishing Drosophila as a valuable model for future studies on EBV and host physiology.