Abstract
Many studies proposed the use of stable carbon isotope ratio (δ(13)C) as a predictor of abiotic stresses in plants, considering only drought and nitrogen deficiency without further investigating the impact of other nutrient deficiencies, that is, phosphorus (P) and/or iron (Fe) deficiencies. To fill this knowledge gap, we assessed the δ(13)C of barley (Hordeum vulgare L.), cucumber (Cucumis sativus L.), maize (Zea mays L.), and tomato (Solanum lycopersicon L.) plants suffering from P, Fe, and combined P/Fe deficiencies during a two-week period using an isotope-ratio mass spectrometer. Simultaneously, plant physiological status was monitored with an infra-red gas analyzer. Results show clear contrasting time-, treatment-, species-, and tissue-specific variations. Furthermore, physiological parameters showed limited correlation with δ(13)C shifts, highlighting that the plants' δ(13)C, does not depend solely on photosynthetic carbon isotope fractionation/discrimination (Δ). Hence, the use of δ(13)C as a predictor is highly discouraged due to its inability to detect and discern different nutrient stresses, especially when combined stresses are present.