Abstract
UV-vis spectrophotometry and spectrofluorometry are indispensable tools in education, research, and industrial process controls with widespread applications in nanoscience encompassing diverse nanomaterials and fields. Nevertheless, the prevailing spectroscopic interpretations and analyses often exhibit ambiguity and errors, particularly evident in the nanoscience literature. This analytical chemistry Perspective focuses on fostering evidence-based data interpretation in experimental studies of materials' UV-vis absorption, scattering, and fluorescence properties. We begin by outlining common issues observed in UV-vis and fluorescence analysis. Subsequently, we provide a summary of recent advances in commercial UV-vis spectrophotometric and spectrofluorometric instruments, emphasizing their potential to enhance scientific rigor in UV-vis and fluorescence analysis. Furthermore, we propose potential avenues for future developments in spectroscopic instrumentation and measurement strategies, aiming to further augment the utility of optical spectroscopy in nano research for samples where optical complexity surpasses existing tools. Through a targeted focus on the critical issues related to UV-vis and fluorescence properties of nanomaterials, this Perspective can serve as a valuable resource for researchers, educators, and practitioners.