Abstract
. is the principal malaria vector in India and has recently developed resistance to synthetic pyrethroids. For identifying the possible operational impact, quantitative measure of the intensity of this resistance is required. The purpose of this study was to measure the intensity of pyrethroid resistance in An. culicifacies s.l. The intensity bioassays with wild caught An. culicifacies s.l were carried out in 10 districts of east central India using 5x and 10x diagnostic concentrations of deltamethrin following WHO insecticides susceptibility guidelines. The results showed that the mortality of An. culicifacies s.l. ranged from 70% to 80% while exposed to 1x DC of deltamethrin (0.05%). Further bioassays conducted with 5x concentration of deltamethrin (0.25%) showed that the mortality of An. culicifacies s.l. in all the 10 districts varied from 92% to 97% indicating moderate resistance. While exposing to 10x concentration (0.5%) of this insecticide, 100% mortality was observed in all the districts; thereby confirmed moderate resistance intensity of this species. Since resistance was not confirmed at the 10x concentration, operational failure of this insecticide was unlikely. Hence, it is recommended to continue the use of LLINs for malaria vector control in these areas.