Handling and Associated Material Properties of Porcine and Human Acellular Dermal Matrices

猪源和人源脱细胞真皮基质的处理及相关材料特性

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Abstract

BACKGROUND: Pliability, handling, and drape characteristics are important material properties of acellular dermal matrices (ADMs). There are few in vitro techniques that quantitatively assess these characteristics. OBJECTIVES: The aim of the authors of this study is to compare data generated using a novel drape assay with surgeon handling of porcine-derived ADMs and evaluate material properties of human- and porcine-derived ADMs. METHODS: Three commercially available ADMs (human-derived AlloDerm [hADM], porcine-derived Strattice Pliable [pADM-S], and porcine-derived Artia [pADM-A]) were assessed. Eight prototype variations of pADM with varied mechanical properties were used for the handling assessment. Drape testing was conducted using a novel mechanical test fixture coupled with quantitative image analysis. Surgeon handling assessments of prototype pADM pliability were compared with quantitative drape measurements to establish a correlation. Commercially available ADMs were tensile tested mechanically at clinically relevant and higher loads to determine maximum strength. RESULTS: Benchtop testing demonstrated similar drapability for pADM-A and hADM; pADM-S had significantly less drapability than pADM-A, despite both being porcine derived. Surgeon semiquantitative ranking of pliability of prototype pADM samples positively correlated with drape measurements (R (2) = 82.5%, P = .000). Tensile testing demonstrated that hADM had the greatest degree of strain/elongation across all applied loads, followed by pADM-A, then pADM-S. When tested to failure, hADM had the greatest average maximum stress compared with pADM-A and pADM-S. CONCLUSIONS: The novel drape assay introduced here correlated with surgeon handling assessment of ADM pliability. Material testing and handling assessment demonstrated various mechanical properties (ie, strength, strain/elongation, drapability, and handling/pliability) for the ADMs evaluated, providing several unique options that may meet different surgical mechanical needs.

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