Abstract
A yeast strain, designated as JAF-11(T), was isolated from flower of Prunus mume Sieb. et Zucc. in Gwangyang, Republic of Korea. Phylogenetic analysis showed that strain JAF-11(T) was closely related to Neodothiora populina CPC 39399(T) with 2.07 % sequence divergence (12 nucleotide substitutions and three gaps in 581 nucleotides) in the D1/D2 domain of the large subunit (LSU) rRNA gene, and Rhizosphaera macrospora CBS 208.79(T) with 4.66 % sequence divergence (25 nucleotide substitutions and five gaps in 535 nucleotides) in the internal transcribed spacer (ITS) region. Further analysis based on the concatenated sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region confirmed that strain JAF-11(T) was well-separated from Neodothiora populina CPC 39399(T). In addition to the phylogenetic differences, strain JAF-11(T) was distinguished from its closest species, Neodothiora populina CPC 39399(T) and Rhizosphaera macrospora CBS 208.79(T) belonging to the family Dothioraceae by its phenotypic characteristics, such as assimilation of carbon sources. Hence, the name Neodothiora pruni sp. nov. is proposed with type strain JAF-11(T) (KACC 48808(T); MB 850034).